PGP9.5 Antibody MA5-29634 from Thermo Fisher Scientific

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IP

PGP9.5 Immunoprecipitation using: Lane A: 0.5 mg 293T Whole Cell Lysate, Lane B: 0.5 mg SH-SY5Y Whole Cell Lysate, Lane C: 0.5 mg U87MG Whole Cell Lysate 2 µL with PGP9.5 Recombinant Rabbit Monoclonal Antibody (104) (Product # MA5-29634) and 15 µL of 50 % Protein G agarose. Primary antibody: PGP9.5 Recombinant Rabbit Monoclonal Antibody (104), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 25 kDa.
ICC/IF

Immunofluorescence staining of PGP9.5 in DU145 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with PGP9.5 Recombinant Rabbit Monoclonal Antibody (104) (Product # MA5-29634, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.

Supplier Page from
Thermo Fisher Scientific for
PGP9.5 Antibody

Thermo Fisher Scientific's PGP9.5 Antibody is a Rabbit monoclonal antibody. The PGP9.5 Antibody was generated using UCHL1 as the antigen. It reacts with Homo Sapiens, and Human. This antibody has been shown to work in applications such as: EIA, Immunoassay, ELISA, Immunocytochemistry, Immunoprecipitation, and Western Blot.

IP

ICC/IF

PGP9.5 Antibody from Thermo Fisher Scientific

Thermo Fisher Scientific

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PGP9.5 Antibody from Thermo Fisher Scientific

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